Journal: Proteomics

Article Title: Evaluation of Quantum dot immunofluorescence and a digital CMOS imaging system as an alternative to conventional organic fluorescence dyes and laser scanning for quantifying protein microarrays

doi: 10.1002/pmic.201500375

Figure Lengend Snippet: Comparison of P3 or Qdot platforms for antibody binding kinetics by protein microarray.

Article Snippet: Conflict of Financial Interest FB, JG, DS and CG are employees of Grace Bio-Labs, Inc. DHD and PLF disclose a financial interest in Antigen Discovery Inc., which has licensed a protein microarray technology.

Techniques: Comparison, Binding Assay, Microarray

Journal: Proteomics

Article Title: Evaluation of Quantum dot immunofluorescence and a digital CMOS imaging system as an alternative to conventional organic fluorescence dyes and laser scanning for quantifying protein microarrays

doi: 10.1002/pmic.201500375

Figure Lengend Snippet: Microarray probing

Article Snippet: Conflict of Financial Interest FB, JG, DS and CG are employees of Grace Bio-Labs, Inc. DHD and PLF disclose a financial interest in Antigen Discovery Inc., which has licensed a protein microarray technology.

Techniques: Microarray

Journal: Microbiology Spectrum

Article Title: Mapping SARS-CoV-2 Antibody Epitopes in COVID-19 Patients with a Multi-Coronavirus Protein Microarray

doi: 10.1128/Spectrum.01416-21

Figure Lengend Snippet: COVID-19 patient and healthy control antibody reactivity with purified SARS-CoV-2 and SARS-CoV proteins. The split violin plot shows the log 2 -transformed fluorescence signal intensity distribution of antibodies bound to each purified protein on the multi-coronavirus protein microarray. Within each half-violin are three lines representing the interquartile range and the median. Above each split violin is the Wilcoxon rank sum P value, colored blue for significant P values below 0.05. The three panels are split by isotype (IgG, top; IgA, middle; IgM, bottom). Horizontal red dashed lines are drawn at the median of all signal intensities against purified proteins ( n = 14) and peptides ( n = 587) plus 1.0, i.e., double the global median; this threshold serves as a point of reference but not necessarily a seropositivity cutoff for each protein.

Article Snippet: The first-generation multi-coronavirus protein microarray, produced by Antigen Discovery, Inc. (ADI, Irvine, CA, USA), included 935 full-length coronavirus proteins, overlapping 100-, 50-, and 30-aa protein fragments, and overlapping 13- to 20-aa peptides from SARS-CoV-2 (WA-1), SARS-CoV, MERS-CoV, HCoV-NL63, and HCoV-OC43.

Techniques: Control, Purification, Transformation Assay, Fluorescence, Microarray

Journal: Microbiology Spectrum

Article Title: Mapping SARS-CoV-2 Antibody Epitopes in COVID-19 Patients with a Multi-Coronavirus Protein Microarray

doi: 10.1128/Spectrum.01416-21

Figure Lengend Snippet: Correlation of IVTT and purified protein microarray results with ELISA and virus neutralization assays. (A to D) The scatterplots show the SARS-CoV-2 S protein-based ELISA Pan Ig signal/threshold ratio ( y axis) plotted against the protein microarray log 2 normalized IgG signal intensity for S2 and N proteins produced in vitro , as well as for the stabilized, purified full-length S protein and the purified RBD fragment of S1 protein ( x axis), respectively. The blue lines were fit to the data using linear regression. (E to H) The dot plots show individual values of each patient for the protein microarray log 2 normalized IgG binding intensity ( y axis) of the four proteins shown in panels A to D at each neutralization titer ( x axis). Red dots are plotted at the means of each stratum, and the red lines represent the 95% confidence intervals.

Article Snippet: The first-generation multi-coronavirus protein microarray, produced by Antigen Discovery, Inc. (ADI, Irvine, CA, USA), included 935 full-length coronavirus proteins, overlapping 100-, 50-, and 30-aa protein fragments, and overlapping 13- to 20-aa peptides from SARS-CoV-2 (WA-1), SARS-CoV, MERS-CoV, HCoV-NL63, and HCoV-OC43.

Techniques: Purification, Microarray, Enzyme-linked Immunosorbent Assay, Virus, Neutralization, Produced, In Vitro, Binding Assay